These are necessary first steps of actual fertilization. The SPA is not an unconditional test for fertility because it does not analyze other aspects of sperm function. What is the sperm penetration assay? In a laboratory sperm is joined with prepared hamster eggs. The number of penetrated eggs is measured to ensure the sperm is undergoing the necessary physiologic changes for fertilization. The hamster eggs are chemically treated to allow normal human sperm to penetrate them.
Microsc Electron Biol Celular. The zona-free hamster oocyte penetration test: a simple procedure using a DNA fluorescent stain to detect the male pronucleus formation. In order to increase the value of the zona-free hamster oocyte penetration test, a comparatively simple and fast method using the fluorochrome Hoechst was developed. Human spermatozoa were washed and incubated 1 hr medium BWW for capacitation.
Thus direct tests of the fertilising capacity of an ejaculate are required in order to assess male infertility. The ideal assessment would be an in vitro fertilisation system incorporating mature human ova. However, because of the obvious logistical and ethical difficulties, such a direct test of fertilising capacity is only possible if some surrogate for the human ovum can be devised. The identification of such a surrogate has been achieved through the elegant studies of Yanagimachi at the University of Hawaii. The key to this discovery was the observation that guinea pig spermatozoa could be induced to penetrate hamster oocytes which had had the zona pellucida removed by trypsin treatment Yanagimachi
Skip to the navigation Test Overview Sperm penetration tests check to see if a man's sperm can move through cervical mucus and the fallopian tubes to join with fertilize an egg. This test is usually done when a couple is having trouble getting pregnant infertility. There are different sperm penetration tests. Sperm mucus penetration. This test checks to see if a man's sperm can move through the cervical mucus.